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Thermo Fisher
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Developmental Studies Hybridoma Bank
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CD107b Antibody [ABL-93] raised in Rat validated in Flow in Mouse.
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Lamp2 rat monoclonal antibody clone ABL 93 APC
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Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay
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Image Search Results
Journal: The Journal of biological chemistry
Article Title: Arrest of mycobacterial phagosome maturation is caused by a block in vesicle fusion between stages controlled by rab5 and rab7.
doi: 10.1074/jbc.272.20.13326
Figure Lengend Snippet: FIG. 6. GTP binding and protein immunoblot analysis of LBC and MPC indicating the presence of rab5 and the absence of rab7 in MPC samples. Equivalent amounts of phagosomes (with the exception of slightly overloaded LBC sample at 168 h) were separated by SDS-PAGE, electroblotted to Immobilon P membranes, and probed successively with [a-32P]GTP (panel A), affinity-purified antibody to rab5a (panel B), affinity-purified antibody against rab7 (panel C), and rat monoclonal antibody to Lamp2 (panel D). Note that the top band of the apparent triplet on the GTP overlay (panel A) corresponds to the location of the polypeptide recognized by the antibody to rab5a.
Article Snippet: For immunoblots, membranes were incubated with affinity-purified antibodies for rab4, rab5a, and rab7 prepared and characterized as described by Zerial et al. (40), or rat monoclonal antibodies to murine Lamp1 (clone 1D4B),
Techniques: Binding Assay, Western Blot, SDS Page, Affinity Purification